Journal: eNeuro
Article Title: Slow NMDA-Mediated Excitation Accelerates Offset-Response Latencies Generated via a Post-Inhibitory Rebound Mechanism
doi: 10.1523/ENEURO.0106-19.2019
Figure Lengend Snippet: Histochemical profile of excitatory and inhibitory inputs to the SPN. A , B , D , E , G , H , Glycinergic input forms the most prominent input to SPN (white dotted circle in A , D , G ) and is depicted by neuronal GlyT2 (green) labeling. Immunolabeling for microtubule associated protein 2 (MAP2, blue) is used as a neuronal marker in all images. Glutamatergic inputs are shown by labeling for the VGLUTs (magenta): VGLUT1 ( A – C ), VGLUT2 ( D – F ), VGLUT3 ( G – I ). A–F , While both VGLUT1 ( B , white arrows) and VGLUT2 ( E , white arrows) positive synaptic boutons are present at the soma, VGLUT2 boutons are also seen in the neuropil ( E , white arrowheads). G–I , VGLUT3 shows only weak somatic but no presynaptic bouton labeling. Scale bars = 200 µm (left images) and 20 µm (middle and right images).
Article Snippet: VGLUT3 , Peptide (C)ELNHEAFVSPRKK, corresponding to amino acid residues 533–545 of rat VGLUT3 (accession Q7TSF2); cytoplasmic, C terminus , Alomone Labs , AGC-037 , Rabbit , 1:300.
Techniques: Labeling, Immunolabeling, Marker